小林研究室 English 東京大学分子細胞生物学研究所へ ENGLISH
研究内容 成果発表 トピックス メンバー紹介 お問い合わせ HOME

成果発表

トップページ > 成果発表 


最近の研究業績(1998~)

Iida, T and Kobayashi, T. (2019) RNA polymerase I activators count and adjust ribosomal RNA gene copy number. Mol. Cell

Hizume, K., Endo, S., Muramatsu, S., Kobayashi, T. and Araki, H. (2018) DNA polymerase ε-dependent modulation of the pausing property of the CMG helicase at the barrier. Genes Dev. 32 1315-1320

Mostofa, G., Rahman, M. A., Koike, N., Yeasmin, A.MST., Islam, N., Waliullah, T.M., Hosoyamada, S., Shimobayashi, M., Kobayashi, T., Hall, M. N., and Ushimaru, T. (2018) CLIP and cohibin separate rDNA from nucleolar proteins destined for degradation by nucleophagy. J. Cell Biol. 217: 2675-2690.

Sasaki, M., and Kobayashi, T. (2017)Ctf4 Prevents Genome Rearrangements by Suppressing DNA Double-Strand Break Formation and Its End Resection at Arrested Replication Forks. Mol. Cell 66:533-545

Kobayashi, T., and Sasaki, M. (2017) rDNA stability is supported by many "Buffer genes" -Introduction to the Yeast rDNA Stability Database. FEMS yeast res. doi: 10.1093/femsyr/fox001

Saka, K., Takahashi, A., Sasaki ,M, and Kobayashi, T. (2016) More than 10% of yeast genes are related to genome stability and influence cellular senescence via rDNA maintenance. Nucleic Acids Res. 44:4211-4221.

Kobayashi, T. (2016) Genome Instability of Repetitive Sequence: Lesson from the Ribosomal RNA Gene Repeat. “DNA Replication, Recombination, and Repair” Hanaoka, F and Sugasawa K. ed. Part IV, Chapter 10: 235-247.

Akamatsu, Y., and Kobayashi, T. (2015) The Human PolI Transcription Terminator Complex Acts as a Replication Fork Barrier that Coordinates the Progress of Replication with rRNA Transcription Activity. Mol Cell Biol. 35, 1871-1881

Kobayashi , T (2014) Ribosomal RNA gene repeats, their stability and cellular senescence. Proc Japan Acad, Ser B, 90, 119-129.

Fawcett, J.A., et al. (2014) Population Genomics of the Fission Yeast Schizosaccharomyces pombe. PLoS One. 9(8):e104241

Saka, K., Ide, S., Ganley, A.R., and Kobayashi, T.  (2013) Cellular Senescence in Yeast Is Regulated by rDNA Noncoding Transcription.  Curr Biol. 23, 1794-1798

Ide, S., Saka, K., and Kobayashi, T.  (2013). Rtt109 prevents hyper-amplification of ribosomal RNA genes through histone modification in budding yeast.  PLoS Genet. 9: e1003410.

Iida, T., Iida, N,. Tsutsui, Y., Yamao, F., and Kobayashi, T.  (2012). RNA interference regulates the cell cycle checkpoint through the RNA export factor, Ptr1, in fission yeast.   BBRC. 427: 143-147.

Poole, A.M., Kobayashi, T., and Ganley, A.R.  (2012). A positive role for yeast extrachromosomal rDNA circles?   Bioessays. 34: 725-729.

Ganley, A.R., Breitenbach, M., Kennedy, B.K., and Kobayashi, T.  (2012). Yeast hypertrophy: cause or consequence of aging? Reply to Bilinski et al . FEMS Yeast Res. 12: 267-268.

Kobayashi, T .  (2011)   . How does genome instability affect lifespan?: roles of rDNA and telomeres.  Genes Cells. 16: 617-624.

Ganley, A.R., and Kobayashi, T. (2011). Monitoring the Rate and Dynamics of Concerted Evolution in the Ribosomal DNA Repeats of Saccharomyces cerevisiae Using Experimental Evolution.   Mol Biol Evol. 28: 2883-2891.  

Miyazaki, T., and Kobayashi, T.  (2011). Visualization of the dynamic behavior of ribosomal RNA gene repeats in living yeast cells.   Genes Cells. 16: 491-502.

Kobayashi, T. (2011). Regulation of ribosomal RNA gene copy number and its role in modulating genome integrity and evolutionary adaptability in yeast. Cell Mol Life Sci. 68: 1395-1403.

Ide, S., and Kobayashi, T. (2010). Analysis of DNA Replication in S. cerevisiae by Two-Dimensional and Pulsed-Field Gel Electrophoresis. Curr. Prot. Sup49 UNIT22.14.1-12.

Ide, S., Miyazaki, T., Maki, H., and Kobayashi, T. (2010). Abundance of ribosomal RNA gene copies maintains genome integrity. Science 327, 693-696.

Ganley, A.R.D., Ide, S., Saka, K., and Kobayashi, T. (2009). The effect of replication initiation on gene amplification in the rDNA and its relationship to aging. Molecular Cell , 35, 683-693.

Iida, T., Nakayama, J. and Moazed, D. (2008). siRNA-mediated heterochromatin establishment requires HP1 and is associated with antisense transcription. Molecular Cell. 31, 178-189.

Kobayashi, T. (2008). A new role of the rDNA and nucleolus in the nucleus- rDNA instability maintains genome integrity-. BioEssays 30, 267-272.

Ganley, A.R.D. and Kobayashi, T. (2007). Phylogenetic footprinting to find functional DNA elements. Methods in Molecular Biology, Comparative Genomics v.1, Humana press, 367-379.

Ganley, A.R.D. and Kobayashi, T. (2007). Highly efficient concerted evolution in the ribosomal DNA repeats: Total rDNA repeat variation revealed by whole-genome shotgun sequence data. Genome Res. 17, 184-191

Ide, S., Watanabe, K., Watanabe, H., Shirahige, K., Kobayashi, T., and Maki, H. (2007). Abnormality in initiation program of DNA replication is monitored by the highly repetitive rRNA gene array on chromosome XII in budding yeast. Mol Cell Biol. 27, 568-578.

Kobayashi, T. (2006). Strategies to maintain the stability of the ribosomal RNA gene repeats. Genes Genet. Syst. 81, 155-161.

Kobayashi. T. and Ganley, A. R. D. (2005). Recombination regulation by transcription-induced cohesin dissociation in rDNA repeats. Science, 309, 1581-1584.

Ganley, A. R. D., Hayashi, K., Horiuchi, T. and Kobayashi, T. (2005). Identifying gene-independent noncoding functional elements in the yeast ribosomal DNA by phylogenetic footprinting. Proc. Natl. Acad. Sci. USA, 102, 11787-11792.

Kobayashi, T., Horiuchi, T., Tongaonkar, P., Vu, L., and Nomura, M. (2004). SIR2 regulates recombination between different rDNA repeats, but not recombination within individual rRNA genes in yeast.Cell, 117, 441-453.

Serizawa, N., Horiuchi, T., and Kobayashi, T. (2004). Transcription-mediated hyper-recombination in HOT1. Genes Cells, 9, 305-315.

Kobayashi, T. (2003). The replication fork barrier site forms a unique structure with Fob1p and inhibits the replication fork. Mol. Cell. Biol. 23, 9178-9188.

Takeuchi, Y., Horiuch, T. and Kobayashi, T. (2003). Transcription-dependent recombination and the role of fork collision in yeast rDNA. Genes Dev. 17, 1497-1506.

Morehouse, E., James, T.Y., Ganley, A.R.D., Vilgalys, R., Berger, L., Murphy, P.J., and Longcore, J.E. (2003). Multilocus sequence typing suggests the chytrid pathogen of amphibians is a recently emerged clone. Molecular Ecology, 12, 395-403.

Kodama, K.-i., Kobayashi, T., Niki, H., Hiraga, S., Oshima, T., Mori, H., and Horiuchi, T. (2002). Amplification of Hot DNA segments in Escherichia coli. Mol. Microbiol. 45, 1575-1588.

Ganley, A.R.D., and Scott, B. (2002). Concerted evolution in the ribosomal RNA genes of an Epichloe endophyte hybrid. Comparison between tandemly-arranged rDNA and dispersed 5S rrn genes. Fungal Genetics and Biology, 35, 39-51.

Wai, H., Johzuka, K., Vu, L., Eliason, K., Kobayashi, T., Horiuchi, T., and Nomura, M.(2001). Yeast RNA polymerase I enhancer is dispensable for transcription of the chromosomal rRNA gene and cell growth, and its apparent transcription enhancement from ectopic promoters required Fob1 protein. Mol. Cell. Biol. 21, 5541-5553.

Kobayashi, T., Nomura, M., and Horiuch, T. (2001). Identification of DNA cis-elements essential for expansion of ribosomal DNA repeats in Saccharomyces cerevisiae: Mol. Cell. Biol 21, 136-147.

Rein, T., Kobayashi, T., Malott, M., Leffak, M., and DePamphilis. M. L. (1999). DNA methylation at mammalian replication origins. J. Biol. Chem. 274, 25792-25800.

Kobayashi, T., Heck, D.J., Nomura, M., and Horiuch, T. (1998). Expansion and contraction of ribosomal DNA repeats in Saccharomyces cerevisiae: requirement of replication fork blocking (Fob1) protein and the role of RNA polymerase I. Genes Dev. 12, 3821-3830.

Ganley, A.R.D. and Scott, B. (1998). Extraordinary ribosomal spacer length heterogeneity in a Neotyphodium endophyte hybrid: Implications for concerted evolution. Genetics, 150, 1625-1637.

Kobayashi, T., Rein, T., and DePamphilis, M. L. (1998). Identification of primary initiation sites for DNA replication in the hamster dihydrofolate reductase gene intiation zone. Mol. Cell. Biol. 18, 3266-3277.

最近の日本語の総説(2001~)

小林武彦(2017)「遺伝学はこんなに面白い!─多様性の学問としての遺伝学」 生物の科学「遺伝」7月号No.4, p308-313
小林武彦(2015) 「本当は楽しいドケチ研究」 実験医学9月号 Vol.33 No.14
小林 武彦(2014) 遺伝子が語る生命38億年の謎―なぜ、ゾウはネズミより長生きか? (共著)国立遺伝学研究所編 悠書館

小林 武彦 (2013)「染色体の安定性の鍵を握る反復配列の維持機構」実験医学vol.31, 2573-2577.

小林 武彦、他、(2013) 「遺伝子図鑑」 国立遺伝学研究所編、編者及び著者、悠書館

小林 武彦、赤松 由布子(2013)「リボソームRNA遺伝子の不安定性と生理作用」生化学、第85巻10号839-844

小林 武彦 他(2013)「岩波生物学辞典」第5版 編者及び著者

小林 武彦 (2012)「rDNA巨大反復遺伝子群による細胞老化制御」実験医学 Vol.30、2228-2233.

小林 武彦 (2012)「今開かれる非コードDNA領域の世界」実験医学 Vol.30、2202-2207.

小林 武彦(2011)「核小体の新機能」生体の科学 vol.62、医学書院、412-415

小林 武彦(2011)「リボソームRNA遺伝子不安定性と細胞老化」生体の科学 vol.62、医学書院、416-417

小林武彦(2010)老化モデル「酵母」新老年学 第3版、2章2.4、東京大学出版会pp265-271

小林 武彦 (2009)「複製、組換え、転写のコラボレーションによる遺伝子増幅」 蛋白質、核酸、酵素、54, 2009, 537-542

小林 武彦 (2009)「リボソームRNA遺伝子の新しい機能」 化学と生物、47・2, 2009, 104-110

小林 武彦(2007)「複製ファークと組換えの共役による遺伝子増幅の制御」実験医学 増刊
Vol.25、635-641.

小林 武彦、Austen R.D. Ganley (2007)「系統発生学的フットプリントによる機能性DNA配列の同定」 遺伝 別冊vo.21、265-268

小林 武彦(2006)「noncodingな転写がrDNAのコピー数を調節する」 蛋白質・核酸・酵素、増刊号『細胞核の世界』、Vol.51、2141-2143.

小林 武彦(2006)「rDNAの恒常性の維持機構」 細胞工学、Vol.25 (5月号)、481?485.

小林 武彦(2006)「酵母の老化制御機構」 医学のあゆみ、217巻、7号、743?747.

小林 武彦(2006)「ゲノムを変化させる遺伝子増幅」 総研大ジャーナル、9号(3月号)、p8-10.

小林 武彦 (2005) 「酵母を使った老化研究?rDNA仮説を中心にー」 基礎老化研究Vol.29、p7-11.

小林 武彦(2004)「SIR2は姉妹染色分体間結合を介してリボゾームRNA遺伝子を安定化している。」 実験医学、vol. 22、pp1835-1838.

小林 武彦、竹内 靖、定塚 勝樹、堀内 嵩(2001)「DNA複製フォークの進行阻害と遺伝子の増幅」 蛋白質・核酸・酵素、vol. 46、pp1004-1012.


 
Copyright 2007 The Kobayashi lab. All rights reserved.